Conversion of Bamboo to Sugars by Dilute Acid and Enzymatic Hydrolysis

Nakorn Tippayawong, Nuttida Chanhom

Abstract


Lignocellulosic biomass is an important alternative energy source to be utilized for ethanol production. In this work, bamboo (Dendrocalamus asper Backer) was used as biomass feedstock for conversion to fermentable sugars. Pretreatment was carried out with dilute sulfuric acid at concentrations between 0.4 – 1.6% w/w, and residence time between 45 – 135 min at a fixed temperature of 140oC. Prehydrolyzate was later analyzed for total sugars by high performance liquid chromatography. For the conditions considered, maximum glucose and xylose yields were obtained from bamboo to be 25.8 and 78.8 mg/mL, respectively. Water insoluble solids obtained were subsequently hydrolysed with cellulase (Trichoderma reesei) and β-glucosidase (Novozyme 188) for 72 h. It was found that increasing yields were obtained with increasing acid concentrations and residence times. The effects of pretreatment severity and enzymatic hydrolysis greatly increased the sugar concentrations after the hydrolysis.

Keywords


Biomass; lignocellulosic; pretreatment; glucose; ethanol; renewable energy

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References


J. Y. Zhu, and X. J. Pan, “Woody biomass pretreatment for cellulosic ethanol production: technology and energy consumption evaluationâ€, Bioresource Technology, vol. 101, pp. 4992–5002, 2010.

W. Leenakul, and N. Tippayawong, “Dilute acid pretreatment of bamboo for ethanol productionâ€, Journal of Sustainable Energy and Environment, vol. 1, pp. 117-120, 2010.

J. M. O. Scurlock, D. C. Dayton, and B. Hames, “Bamboo: an overlooked biomass resource?â€, Biomass and Bioenergy, vol. 19, pp. 229-244, 2000.

A. T. W. Hendriks, and G. Zeeman, “Pretreatments to enhance the digestibility of lignocellulosic biomassâ€, Bioresource Technology, vol. 80, pp. 1-9, 2008.

T. Shimokawa, M. Ishida, S. Yoshida, and M. Nojiri, “Effects of growth stage on enzymatic saccharification and simultaneous saccharification and fermentation of bamboo shoots for bioethanol productionâ€, Bioresource Technology, vol. 100, pp. 6651–6654, 2009.

N. Sathitsuksanoh, Z. Zhu, T. Hoc, M. Bai, Y. Heng, and P. Zhang, “Bamboo saccharification through cellulose solvent-based biomass pretreatmentfollowed by enzymatic hydrolysis at ultra-low cellulase loadingsâ€, Bioresource Technology, vol. 101, pp. 4926–4929, 2010.

I. Ballesteros, M. Ballesteros, P. Manzanares, and M. J. Negro, “Dilute sulfuric acid pretreatment of cardoon for ethanol production, Biochemical Engineering Journal, vol. 60, pp. 84–91, 2008.

I. D. Campo, M. Z. Alegr, M. Echeverr, and I. Echeverr, “Diluted acid hydrolysis pretreatment of agri-food wastes for bioethanol productionâ€, Industrial Crops and Product, vol. 24, pp. 214–221, 2006.

Y. Sun, and J. J. Cheng, “Dilute sulfuric acid pretreatment of rye straw and bermudagrass for ethanol productionâ€, Bioresourse Technology, vol. 96, pp. 1599-1606, 2005.

T. A. Lloyd, and C. E. Wyman, “Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solidsâ€, Bioresource Technology, vol. 96, pp. 1-11, 2005.

C. Cara, E. Ruiz, J. M. Oliva, F. Saez, and E. Castro, “Conversion of olive tree biomass into fermentable sugars by diluteacid pretreatment and enzymatic saccharificationâ€, Bioresource Technology, vol. 99, pp. 1869–1876, 2007.

G. L. Guo, W. H. Chen, W. H. Chen, L. C. Men, and W. S. Hwang, “Characterization of dilute acid pretreatment of silvergrass for ethanol productionâ€, Bioresource Technology, vol. 99, pp. 6046–6053, 2008.

C. E. Wyman, B. E. Dale, R. T. Elander, M. Holtzapple, M. R. Ladisch, Y. Y. Lee, C. Mitchinson, and J. N. Saddler, “Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologiesâ€, Biotechnology Progress, vol. 25, pp. 333–339, 2009.

W. Zhu, J. Y. Zhu., R. Gleisner, and X. J. Pan, “On energy consumption for size reduction and enzymatic hydrolysis of lodgepole pineâ€, Bioresource Technology, vol. 101, pp. 2782–2792, 2010.

Y. Chen, B. Dong, W. Qin, and D. Xiao “Xylose and cellulose fractionation from corncob with three different strategies and separate fermentation of them to bioethanolâ€, Bioresource Technology, vol. 101, pp. 6994–6999, 2010.




DOI (PDF): https://doi.org/10.20508/ijrer.v1i4.74.g79

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